InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay

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The InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay measures protein binding via compound-induced binding partner disruption and degradation. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 96-0009E3CP16L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cells included overexpress Bcl2L1 target in an untagged form and Bax binding partner fused to PL. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Family:
BCL2
Accession Number:
NM_138578.1
Target Name:
Bcl2L1-Bax
Target Aliases:
BCL2-like 1
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Target Engagement
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Target Engagement
Detection Method:
Chemiluminescence
Additional Information
Brand:
InCELL Hunter™

User Manuals & Protocols

70-429 InCELL Hunter eXpress Assays User Manual REV0A

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Datasheets

96-0009E3x Datasheet

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